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DMPO: Biotin

DMPO: Biotin

Cat no: SMC-191D


Supplier: StressMarq Biosciences Inc.
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Detects ~90kDa. Recognzies DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.
Catalogue number: SMC-191D
Hosts: Mouse
Applications: Immunoprecipitation, Western Blot
Size: 100ug
Clone: N1664A
Form: Protein G Purified
Storage buffer: TBS pH7.4, 0.05% sodium azide, 50% glycerol
Concentration: 1mg/mL
Isotype: IgG
Shipping temp: Blue Ice or 4
Storage temp: -20
Research area: Oxidative Stress
Certificate of analysis: A 1/1000 dilution of SMC-191 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Scientific background: The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states (1). The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-
Alternative names: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N oxide
References: 1. Reszka K.J., et al. (2006) Nitric Oxide 15: 133-141. 2. Ramirez D.C., Gomez-Mejiba S.E., and Mason R.P. (2007) Nat Protoc. 2(3): 512-522. 3. Khan N., et al. (2003) Free Radic. Biol. Med 34:1473