logo
logo
cDNA Library S.pombe Meiosis

Cat no: 02-705

cDNA Library S.pombe Meiosis

This cDNA library (plasmid DNA) is constructed from Schizosaccharomyces pombe strain CD16-1(h+/h-) derived poly(A)+ RNA at the state of miosis by the Linker-Primer method (Ref.1) by Professor Hiroshi Nojima of Research Institute for Microbial Diseases Osaka University. cDNAs in this library are unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (Bgl II)-Sma I adaptor. The pAP3neo vector used in this library can express the S. pombe genes in mammalian cells as it contains SV40 promoter. It also contains f1 ori which is necessary for ssDNA synthesis and bacteriophage T7 and T3 promoter for RNA synthesis (see Figure). GenBank Accession No. AB003468 Application: PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein productions and preparation of probes etc. Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression levels of mRNA of the genes.)

Prices direct from B-BRIDGE INTERNATIONAL, INC

Quick response times

Exclusive Absave savings/discounts

SPECIFICATIONS

Catalog Number

02-705

Size

500 ng

Storage Temp

- 20 degree C

Shipping Temp

4C

Latest promotions

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

Buy any polyclonal or monoclonal antibody from our extensive range of pre-made antibodies and for a limited time only receive a $50 discount!(T&C apply:...

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...