Blocking Buffer for Fluorescent Western Blotting

Fluorescence technology is widely used to detect proteins in both the visible and near-infrared ranges. This product allows for superior signal detection and lower background noise when fluorochrome conjugated antibodies are used to visualize proteins in western blotting and other applications. Antibody conjugates prepared with IRDye(R) 800 and IRDye(R) 700DX (Licor), Cy2(TM), Cy3(TM), Cy3.5(TM), Cy5(TM) and Cy5.5(TM) (GE Healthcare), DyLight(TM)405, DyLight(TM) 549, DyLight(TM) 649, DyLight(TM) 680, and DyLight(TM) 800 (Thermo Fisher/Pierce) and Alexa Fluor(R) 488, Alexa Fluor(R) 532, Alexa Fluor(R) 546, Alexa Fluor(R) 647 and Alexa Fluor(R) 680 (Invitrogen/Molecular Probes) have been validated on various platforms using this product with superior results compared to other commercially available products. In the infrared range, where little to no autofluorescence occurs, specific signal is sharply evident from any background giving the best possible signal-to-noise ratio. This allows for detection levels in the picogram range which rivals the sensitivity of chemiluminescence on film for western blotting. Superior results are also seen when this product is used for simultaneous labeling (multiplex) in western blots or microscopy using various fluorochrome combinations for multicolor imaging. Membranes blocked with this product can be dried and are very stable. Membranes that are stored protected from light can be re-washed and/or rescanned.