anti-hu CD138 pluriBeads

PluriBead is a unique and robust non-magnetic separation technology for cells, proteins, and other targets from nearly all biological fluids. The procedure is simple: You sieve your pluriBeads bound to target cells through a strainer - the pluriBeads with your target cells stay on top, the unwanted cells pass through the strainer. Then gentle detachment of your cells with a buffer is all you do, the target cells are ready to use! PluriBeads are coated with an antibody against a specific target marker. Use the pluriBeads for positive selection or negative depletion of a cell population. Bead Size: M Volume: 2 ml Target Marker: CD138 protein, in humans, is encoded by the SDC1 gene. CD138 is a heparin sulfate proteoglycan is a membrane protein that participates in cell proliferation, cell migration, and cell-matrix interactions. Species: Human Target Cells:Plasma Cells (plasma B cells, plasmocytes, effector B cells) Sample Type: PBMC, blood, primary cell solution (also review definitions below) Starting Material: 200 ul blood or 10^5 cells per isolation Capacity: 20 ul for maximum of 5 x10^5 target cells Output Viability: Greater than 95 percent Output Purity: Greater than 96 percent _____________ Definitions/Applications/Components: S-pluriBeads: 30 um diameter 10^7 maximum target cells per separation 400 ul maximum bead suspension per strainer Sample material – whole blood, tissue, PBMC, cell culture Minimum sample volume is 200 ul Recommended Applications for small number of target cells in a large sample volume and for Circulating Tumor Cells (CTC) M-pluriBeads: 60 um diameter 5 x 10^7 maximum target cells per separation 1000 ul maximum bead suspension per strainer Sample material – whole blood, tissue, PBMC, cell culture, and buffy coat Minimum sample volume is 500 ul Recommended Applications for large number of target cells in sample